ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Objective To study the genotype of Yersinia pestis in Hebei plague foci by variable number of tandem repeat (VNTR). Methods Primers were designed according to the confirmed 14+12 VNTR, to genotype the 116 Y. pestis DNA of Hebei province. Results All of the strains showed one genetype, but they were different from CO92 and EV. Conclusion There is only one genetype of plague, indicating a genetic stability in Y. pestis in Hebei province.
The plague is categorized as a Class A infectious disease in China. Plague foci are widely distributed, and strains have different features and varied virulence in China. It is of great significance for deducing the sudden outbreak of plague and terrorist attack detection, with the strains of different foci for genotyping. The choice of tandem repeats loci is the key of the multiple loci vntr analysis (MLVA) classification results. This assay mainly introduces tandem repeats in Yersinia pestis genotyping in application progress, and provides a reference for other workers in genotyping.
Objective To update the information on the species of ectoparasitic fleas on small mammals in Gonghe county, Qinghai province, China. Methods Small mammals were captured by night trapping method, and ectoparasitic fleas were collected from them. The slide specimens of fleas were made, and these fleas were classified and identified based on morphological characteristics. Results A total of 785 ectoparasitic fleas (13 species, 8 genera, 3 families) were collected from small mammals in Gonghe county. Conclusion Neopsylla bidentatiformis (Wagner, 1883) is a new record in Qinghai province.
【Abstract】 Objective To study the practicability of double antigens sandwich enzyme linked immunosorbent assay(DAgS?ELISA)on the detection of Yersinia pestis F1 antibodies. Methods A total of 558 samples antibodies of anti?F1 antigen were detected by DAgS?ELISA and trace indirect hemagglutination assay (trace?IHA). Results Thirty three samples were positive tested by IHA, 31 positive by DAgS?ELISA, the positive accordance rate was 90.91%, 99.81% for negative accordance rate, 99.28% for the total accordance rate. The positive rate detected by IHA and DAgS?ELISA were 5.91% and 5.56% respectively, and no statistics difference was found (χ2=0.25,P=0.625). About 27 the immuno?serum were positive detected by IHA and DAgS?ELISA methods, and the sensitivity of IHA test were all higher than that of DAgS?ELISA (t=3.023, P=0.006). Conclusion Sensitivity of DAgS?ELISA is lower than that of trace?IHA, but its specificity is better and no primary inhibitory phenomena, and could exempt from leak detection in the preliminary screening.